Abstract
Acheilognathus tonkinensis is a magnificent bitterling fish of genus Acheilognathus belonging to the sub-family Acheilognathinae of the family Cyprinidae. In this study, we first determined and described the 16,767 bp mitochondrial DNA sequence of A. tonkinensis. The mitogenome encoded 13 protein-coding genes (PCGs), 22 tRNA genes, 12S, and 16S rRNA genes, and a D-loop region. The overall nucleotide composition was 29.4% A, 27.1% T, 17.0% G, and 26.5% C, with a slight AT bias (56.5%). Phylogenetic analysis suggested that A. tonkinensis had the closest evolutionary relationship with A. macropterus. The availability of mitogenome sequence of A. tonkinensis would facilitate species identification of the Acheilognathinae, as well as genetic evaluations for resource conservation and management of this species.