Methods
following the hydrolysis of o-nitrophenyl-β-d-galactopyranoside and of 5-bromo-4-chloro-3-indoyl-β-d-galactopyranoside (X-gal) in tryptic soy agar plates. The relative expression of the β-galactosidase gene was performed using real-time quantitative PCR. Despite generally lower growth in AW, 18 strains showed higher β-galactosidase activity when grown in AW compared with MRS medium. The highest β-galactosidase activity in AW was in Lactobacillus helveticus strain OSU-PECh-4A, which showed almost 5 times higher activity than average. Analysis of 6 selected strains for expression of the bgal-620 gene found higher overexpression in AW than in MRS, regardless of specific β-galactosidase activity. Strains of LAB such as OSU-PECh-4A could valorize AW through the production of β-galactosidase (as an aid to lactose digestion) and production of prebiotic galactooligosaccharides.