Background
Derivation of induced pluripotent stem cells (iPSCs) from various adult somatic cells through over-expression of pluripotent genes could allow for the unlimited autologous supply in regenerative medicine. On the other hand the generation of various progenitors from bone-marrow mesenchymal stem cells (MSCs) is justly well established.
Conclusion
It seems that the higher expression of osteopontin and osteocalcin in MSCs compared to iPSCs may be due to other factors (besides pluripotency) required for differentiation of stem cells to osteoblast.
Methods
We analyzed the expression level of oct4, sox-2, c-myc, klf4, nanog and lin28 genes in human MSCs derived from iPSCs and MSCs by cell culture and real-time PCR. Also the expression level of osteocalcin and osteopontin in both groups were evaluated.
Objective
In this study we compared the expression level of pluripotent genes oct4, c-myc, sox-2, nanog, klf4 and lin28 in iPSCs and MSCs derived from bone marrow. Also the potential of osteogenesis of iPSCs and bone-marrow MSCs were compared.
Results
We found that the expression of osteogenic markers in differentiated iPSCs to osteoblast were higher than bone-marrow MSCs. While the levels of pluripotency genes oct4, c-myc and klf4 in iPSCs were significantly (p<0.05) higher than bone-marrow MSCs, MSCs showed higher expression of sox-2, nanog and lin28 compared with iPSCs (p=NS).