Abstract
Glioma is a type of malignant tumor accounting for 80% of all brain cancer morbidity. The long non-coding RNA (lncRNA) PVT1 has been demonstrated to be an oncogenic lncRNA in other types of cancer. However, the role of PVT1 in glioma is still unknown. The aim of the present study was to investigate the role of PVT1 in glioma, and its potential association with microRNA (miR)-200a. miR-200a mimics and small interfering (si)RNA transfection were utilized to construct miR-200a overexpression and knockdown models to investigate the effect of miR-200a on glioma cells. Slow-virus infection was used to transfect cells. Western blotting and reverse transcription-quantitative polymerase chain reaction were applied for the quantitative analysis of mRNA and protein expression. Apoptosis of podocytes was detected by terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling staining. PVT1 expression in glioma was upregulated. In vitro, PVT1 silencing via transfection with si-PVT1 suppressed proliferation and invasion and induced G0/G1 phase arrest. Luciferase reporter assay revealed the association between miR-200a and the PVT1 3'-untranslated region. Furthermore, experiments examining both miR-200a and PVT1 indicated that miR-200a could reverse the effects of PVT1 on glioma cell phenotypes. The present study reveals the overexpression of PVT1 in glioma tissue and cells and the oncogenic role of PVT1 in gliomagenesis via sponging miR-200a, thus providing a potential biomarker for the early detection of glioma and prognosis prediction.