Abstract
In this study, we constructed a new fluorescent sensing for VB(12) and investigated the mechanism of vitamin B(12) (VB(12)) quenching fluorescence of carbon dots (CDs). The fluorescence suppression is attributed to the inner filter effect (IFE) because of the overlap between UV-vis absorption spectrum of VB(12) and emission/excitation spectra of CDs. This CDs-based sensor provides obvious advantages of simplicity, convenience, rapid response, high selectivity and sensitivity, which has potential application for the detection of VB(12) in the medical and food industry.