Abstract
BACKGROUND: Accurate and complete response evaluation after treatment is important to implement individualized therapy for gastric cancer. PURPOSE: To investigate the effectiveness of diffusion kurtosis imaging (DKI) and in-line X-ray phase contrast imaging (ILXPCI) in the assessment of the therapeutic efficacy by transforming growth factor beta 1 (TGF-β1) inhibition. STUDY TYPE: Prospective animal study. ANIMAL MODEL: Thirty nude mice subcutaneous xenotransplantation tumor model of gastric cancer for DKI and 10 peritoneal metastasis nude mice model for ILXPCI. FIELD STRENGTH/SEQUENCE: Examinations before and serially at 7, 14, 21, and 28 days after TGF-β1 inhibition treatment were performed at 3T MRI including T(2) -weighted imaging (T(2) WI) and DKI with five b values of 0, 500, 1000, 1500, 2000 s/mm(2) ; ILXPCI examinations were performed at 14 days after treatment. ASSESSMENT: DKI parameters (apparent diffusion coefficient [ADC], diffusivity [D] and kurtosis [K]) were calculated by two experienced radiologists after postprocessing. STATISTICAL TESTS: For the differences in all the parameters between the baseline and each timepoint for both the treated and the control mice, the Mann-Whitney test was used. The Spearman correlation test was used to evaluate correlations among the DKI parameters and corresponding pathologic necrosis fraction (NF). RESULTS: ADC, D, and K values were significantly different between the two groups after treatment (P < 0.05). Serial measurements in the treated group showed that the ADC, D, and K values were significantly different at 7, 14, 21, and 28 days compared with baseline (P < 0.05). There were significant correlations between DKI parameters and NF (ADC, r = 0.865, P < 0.001; D, r = 0.802, P < 0.001; K, r = -0.944, P < 0.001). The ILXPCI results in the treated group showed a stronger absorption area than the control group. DATA CONCLUSION: DKI may be used to evaluate the complete course therapeutic effects of gastric cancer induced by TGF-β1 inhibition, and the ILXPCI technique will improve the tumor microstructure resolution. LEVEL OF EVIDENCE: 1 Technical Efficacy: Stage 4 J. Magn. Reson. Imaging 2019;49:1553-1564.