Ultrasensitive determination of ascorbic acid by using cobalt oxyhydroxide nanosheets to enhance the chemiluminescence of the luminol-H(2)O(2) system

利用氢氧化钴纳米片增强鲁米诺-H₂O₂体系的化学发光,实现抗坏血酸的超灵敏测定。

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Abstract

Ascorbic acid (AA) as an essential vitamin in the human body participates in various physiological reactions and plays a key role in many biochemical processes. Therefore, it is of vital importance to monitor and quantify AA in commercial tablets, beverages and food. In this work, a rapid and ultrasensitive chemiluminescence (CL) system for the detection of AA was developed, in which ultrathin cobalt oxyhydroxide (CoOOH) nanosheets were applied in the conventional luminol-H(2)O(2) CL system. The results showed that ultrathin CoOOH nanosheets as a catalyzer remarkably improved the CL intensity of the CoOOH-luminol-H(2)O(2) system, up to about 1400-fold. Under the optimized conditions, the CL inhibition efficiencies increase linearly with the concentrations of AA in the range of 1-500 pmol L(-1), and the limit of detection was 39 fmol L(-1). Moreover, the proposed CL system was successfully applied in the determination of AA in medicinal tablets with satisfactory results.

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