Abstract
Lonicera microphylla Willd. ex Roem. & Schult. 1819 is a medicinal plant species, especially for treating chronic fever and dysentery. This study aimed to characterize the chloroplast genome of L. microphylla and reconstruct the phylogenetic relationships among Lonicera Linn. 1753. The circular complete chloroplast genome is 154,945 bp in length, with two inverted repeat regions (IRs; 23,841 bp), separated by a small single-copy region (SSC; 18,789 bp) and a large single-copy region (LSC; 88,474 bp). The overall GC content of L. microphylla plastome was 38.36%. The chloroplast genome encoded a total of 131 genes, including 84 protein-coding genes, 39 tRNA genes, and eight rRNA genes. Phylogenetic analysis reveals that L. microphylla was more closely related to Lonicera tangutica Maxim. 1878. Our result can provide reference for the phylogenetic relationship, resource development, and utilization of Lonicera.