Abstract
A polyphasic taxonomic study was carried out on the rod-shaped, orange-pigmented strain C11(T), isolated from gold mine tailings. Sequencing of the 16S rRNA gene showed a relatedness to Brevundimonas, with a 98.4% and 98.2% similarity to Brevundimonas bacteroides and Brevundimonas variabilis, respectively. The average nucleotide identity and a digital DNA-DNA hybridization with the closest phylogenetic neighbor of strain C11(T) indicate distinction at the species level, further confirmed by the differences in physiology. C(18:1) ω7c is the dominant cellular fatty acid. Its DNA G + C content is 68.3 mol %. Its predominant ubiquinone is Q-10; 1,2-Di-O-acyl-3-O-α-D-glucopyranuronosyl glycerol, phosphatidylglycerol, 1,2-di-O-acyl-3-O-α-D-glucopyranosyl glycerol, and 1,2-di-O-acyl-3-O-[D-glucopyranosyl-(1→4)-α-D-glucopyranuronosyl] glycerol are its major polar lipid constituents. This bacterium produces bacteriochlorophyll a and tolerates high concentrations of (μg/mL) the following: tellurium (>1500), selenium (1000 to >5000), and vanadium (>5000) oxyanions. The data support the inclusion of the strain C11(T) into the genus Brevundimonas as a new species with the proposed name Brevundimonas aurifodinae sp. nov. (C11(T) = NRRL B-65718(T); =DSM 118059(T)).