Abstract
BACKGROUND: Methyl acetate (MA) is a common industrial solvent that causes rapid blindness in large exposures. Its toxicologic mechanism is not fully elucidated currently. The currently used clinical marker for MA poisoning, formic acid, is unable to differentiate between MA exposure and methanol exposure, which hinders accurate diagnosis and exposure source tracing, and impairs the development and implementation of front-end preventive and control measures. OBJECTIVE: This study utilized a cross-species, untargeted metabolomics approach, combining data from animal models and human cohorts, aiming to identify potential biomarkers for MA poisoning and provide new insights into its toxicological mechanisms. METHODS: Subacute poisoning rat models of MA and methanol were established via gavage administration (n = 6 per group) and urine samples were collected. Meanwhile, 8 occupationally exposed MA-intoxicated patients and 10 healthy controls were enrolled, with their urine samples also being collected. All samples underwent untargeted metabolomic analysis using UPLC-QTOF/MS for comparative profiling among MA-exposed rats versus control rats, MA-exposed rats versus methanol-exposed rats, and MA-exposed patients versus healthy controls. RESULTS: A total of 41 and 16 significantly altered metabolites were identified in MA-exposed rat models and occupationally exposed human subjects, respectively. Pathway enrichment analysis further revealed key pathways including the tricarboxylic acid (TCA) cycle, purine metabolism, glutathione metabolism, cysteine and methionine metabolism, and one-carbon metabolism, suggesting conservation of MA-induced toxic responses across species. These results indicate that MA toxicity involves not only classical TCA cycle inhibition but also close association with systemic oxidative stress. 20-carboxy-leukotrieneB(4) (20-COOH-LTB(4)) and S-adenosylhomocysteine (SAH) were significantly elevated in the MA exposure group in both rat and human samples, but were not detected in the methanol exposure group, showing high specificity and cross-species conservation. CONCLUSION: This study reveals MA toxicity mechanism via oxidative stress, aids in developing therapies and enhancing MA exposure risk management. And the study identifies 20 - COOH - LTB(4) and SAH as potential and sensitive biomarkers for MA intoxication, offering a tool for differentiating MA from methanol exposure clinically.