Abstract
A novel Rhodococcus pyridinivorans strain FL1, capable of degrading aniline, was isolated from an urban river ecosystem. The complete genome sequence of this strain was presented, and the key enzymes involved in the aniline degradation pathway, including catechol 1,2-dioxygenase (catA) and 3-carboxy-cis,cis-muconate cycloisomerase, were systematically annotated.