Modeling autoregulation of cardiac excitation-Ca-contraction and arrhythmogenic activities in response to mechanical load changes

模拟心脏兴奋-钙收缩和心律失常活动对机械负荷变化的自动调节

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Abstract

The heart has intrinsic abilities to autoregulate contractile force in response to mechanical load. Recent experimental studies show that cardiomyocytes have mechano-chemo-transduction (MCT) mechanisms that form a closed feedback loop in the excitation-Ca(2+) signaling-contraction (E-C) coupling. This closed feedback loop enables autoregulation of contraction in response to mechanical load changes. Here, we develop the first autoregulatory E-C coupling model that couples electrophysiology, Ca(2+) signaling, force development and contraction, and MCT feedback. The model recapitulates the experimental data showing that the mechanical load on cardiomyocytes during contraction increases the L-type Ca(2+) current, action potential duration, sarcoplasmic reticulum (SR) Ca(2+) content, and SR Ca(2+) release, giving rise to increased cytosolic Ca(2+) transient (MCT-Ca(2+) gain) and enhanced contraction. The model also makes non-trivial predictions on the autoregulation of contraction with moderate MCT-Ca(2+) gain under a range of physiological load changes, but arrhythmogenic discordant alternans with excessive MCT-Ca(2+) gain under pathological overload.

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