Abstract
This study attempts to construct a robust model that describes the interaction between low-intensity laser irradiation (LILI) and its effects on platelet aggregation through various mechanisms using bioavailable nitric oxide and selective markers for activation. These findings will assist in the improvement of therapeutic techniques intended for the treatment of disease of improper platelet function. Fifty blood samples from healthy individuals aged 20 to 45 years were collected. The main focus was to investigate the dose-aggregation response of platelets on stimulation by three agonists, i.e., thrombin receptor-activating peptide, collagen, and adenosine diphosphate (ADP). The responses of platelets were assessed after stimulation with LILI of wavelength λ = 830 nm. LILI treatment caused a significant reduction of platelet aggregation at all doses tested; nevertheless, the most pronounced effect was apparent at the dose of 9.5 J/cm². There were no differences in concentrations of compounds related to the nitric oxide metabolic pathway or platelet activation markers found between the tested groups. With the application of a highly validated in vitro model, LILI is uniform in inhibiting whole blood platelet aggregates without necessitating alterations in activation markers in platelets or in nitric oxide permeability. This observation indicates the therapeutic potential of LILI as an agent in the modulation of platelet aggregation with maintenance of normal physiological response.