Abstract
PURPOSE: This work was performed to investigate the effects of tissue microstructure from postmortem rat brain on the shape of the water proton spectrum. METHODS: Perfusion-fixed, resected rat brains (N = 4) were imaged at 9.4T. 3D DTI and 3D echo-planar spectroscopic imaging (EPSI) data were acquired with 150 μm isotropic resolution. DTI data were acquired over 60 directions with b = 3000 s/mm(2) . Water spectra were produced from EPSI data acquired over 128 echoes, with 2.9 Hz spectral resolution. A voxel-wise metric reflecting spectral asymmetry about the peak of the resonance was computed and compared with orientation estimates from DTI data by fitting data with the susceptibility anisotropy model. RESULTS: Asymmetric broadening of the water resonance was computed for mixed populations of grey and/or white matter as determined by thresholding the fractional anisotropy. Asymmetry was shown to be differentially affected by tract orientation relative to B(0) in high FA voxels, whereas low FA voxels exhibited little sensitivity. Anatomic structures in the hippocampus were also found to produce distinct changes in the water resonance. CONCLUSION: Present results demonstrate that structural variations in tissue architecture cause characteristic, reproducible changes in the water resonance shape. This suggests that water spectra are sensitive to cytoarchitectural variations in brain tissue.