Abstract
BACKGROUND: While graph measures are used increasingly to characterize human connectomes, uncertainty remains in how to use these metrics in a quantitative and reproducible manner. Specifically, there is a lack of community consensus regarding the number of streamlines needed to generate connectomes. PURPOSE: The purpose was to define the relationship between streamline count and graph-measure value, reproducibility, and repeatability. STUDY TYPE: Retrospective analysis of previously prospective study. POPULATION: Ten healthy subjects, 70% female, aged 25.3 ± 5.9 years. FIELD STRENGTH/SEQUENCE: A 3-T, T1-weighted sequences and diffusion-weighted imaging (DWI) with two gradient strengths (b-values = 1200 and 3000 sec/mm(2) , echo time [TE] = 68 msec, repetition time [TR] = 5.4 seconds, 120 slices, field of view = 188 mm(2) ). ASSESSMENT: A total of 13 graph-theory measures were derived for each subject by generating probabilistic whole-brain tractography from DWI and mapping the structural connectivity to connectomes. The streamline count invariance from changes in mean, repeatability, and reproducibility were derived. STATISTICAL TESTS: Paired t-test with P value <0.05 was used to compare graph-measure means with a reference, intraclass correlation coefficient (ICC) to measure repeatability, and concordance correlation coefficient (CCC) to measure reproducibility. RESULTS: Modularity and global efficiency converged to their reference mean with ICC > 0.90 and CCC > 0.99. Edge count, small-worldness, randomness, and average betweenness centrality converged to the reference mean, with ICC > 0.90 and CCC > 0.95. Assortativity and average participation coefficient converged with ICC > 0.75 and CCC > 0.90. Density, average node strength, average node degree, characteristic path length, average local efficiency, and average clustering coefficient did not converge, though had ICC > 0.90 and CCC > 0.99. For these measures, alternate definitions that converge a reference mean are provided. DATA CONCLUSION: Modularity and global efficiency are streamline count invariant for greater than 6 million and 100,000 streamlines, respectively. Density, average node strength, average node degree, characteristic path length, average local efficiency, and average clustering coefficient were strongly dependent on streamline count. EVIDENCE LEVEL: 1. TECHNICAL EFFICACY: Stage 1.