Marker removal system for Thermoanaerobacterium saccharolyticum and development of a markerless ethanologen
解糖热厌氧杆菌的标记物去除系统及无标记乙醇产菌的开发
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作者：A Joe Shaw, Sean F Covalla, David A Hogsett, Christopher D Herring
Abstract
Marker removal strategies were developed for Thermoanaerobacterium saccharolyticum to select against the pyrF gene and the pta and ack genes. The pta- and ack-based haloacetate selective strategy was subsequently used to create strain M0355, a markerless Δldh Δpta Δack strain that produces ethanol at a high yield.
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