Abstract
We present a comprehensive theoretical-experimental framework for quantitative, high-throughput study of cell biomechanics. An improved electrodeformation method has been developed by combing dielectrophoresis and amplitude shift keying, a form of amplitude modulation. This method offers a potential to fully control the magnitude and rate of deformation in cell membranes. In healthy human red blood cells, nonlinear viscoelasticity of cell membranes is obtained through variable amplitude load testing. A mathematical model to predict cellular deformations is validated using the experimental results of healthy human red blood cells subjected to various types of loading. These results demonstrate new capabilities of the electrodeformation technique and the validated mathematical model to explore the effects of different loading configurations on the cellular mechanical behavior. This gives it more advantages over existing methods and can be further developed to study the effects of strain rate and loading waveform on the mechanical properties of biological cells in health and disease.