Abstract
PURPOSE: To explore the mechanisms by which abnormal female BMI affects oocyte quality, particularly whether it involves the alteration of gene expression patterns and how these patterns may impact clinical outcomes. METHODS: In Part 1, we performed a retrospective study to compare the clinical outcomes between the female BMI ≥25 kg/m(2) and female BMI ≤20 kg/m(2) groups. In Part 2, we performed the transcriptome analyses based on the GSE87201 dataset. RESULTS: In Part 1, among the clinical outcomes, only the grade 1-2 embryo rate at day 3 of ICSI cycles was significantly different between the two BMI groups; the other outcomes were not. In Part 2, compared with the BMI ≤20 kg/m(2) group, the oocyte gene expression pattern of the BMI ≥25 kg/m(2) group seemed to result in better oocyte tolerance to exogenous stress, such as intracytoplasmic sperm injection (ICSI). It seemed to explain the result of Part 1 that the BMI ≥25 kg/m(2) group had better day-3 embryo quality after ICSI than the BMI ≤20 kg/m(2) group. CONCLUSIONS: Abnormal female BMI affects oocyte quality by altering the gene expression patterns of oocytes. While a female BMI ≥25 kg/m(2) is known to have certain detrimental effects on ART, our findings suggest that it can also confer some benefits to oocytes.