Cappable-seq RNA-sequencing data sets of Escherichia coli K-12 MG1655 treated with novobiocin, tetracycline, and rifampicin

Mapping transcription start sites and determining their activity remain a challenging task even for well-studied organisms. Here, we present Cappable-seq RNA sequencing data of Escherichia coli K-12 MG1655 after treatment with three antibiotics with various spectra of action that may expand the range of mapped transcription start sites for this model organism.