Abstract
The removal of Direct Blue 71 (DB71), by laccase (EC 1.10.3.2, p-bezenediol:dioxygen oxidoreductases) enzyme in presence of 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), in aqueous solution was investigated. Data on this article focused on the optimizing and modeling of DB71 removal by Response surface method (RSM) based on Box-Behnken design (BBD), through studying the effective variables as follows: ABTS dose (0.05-0.2 mM), Laccase dose (0.05-0.2 U mL(-1)), and pH (3-7). The results of experimental showed that laccase was able to removal DB71 with removal percentage of 83% at concentration of 50 mg L(-1) after 30 min incubation in presence of ABTS 0.2 mM, at temperature 40 °C and pH 5. The Analysis of Variance (ANOVA) for the predicted quadratic model was done and quadratic equation showed significant R-Squared (0.9969), Adjusted R-Squared (0.9914) and Adequate Precision (49.7). The lack of fit is not significant due to p-value prob > F more than 0.05.