Abstract
Here, we describe a Bacillus subtilis bacteriophage isolated from sewage water. The phage fHSPT3 was isolated against the host Bacillus subtilis 168 and has a genome size of 150,187 bp with 221 protein-coding sequences. The fHSPT3 belongs to the genus Siophivirus, lacks virulence or antibiotic resistance genes, and shows a virulent life cycle predicted through PhageScope and PhageAI.