Abstract
Oligodeoxynucleotides (ODNs) containing unmethylated cytosine-phosphate-guanosine (CpG) motifs are readily recognized by Toll-like receptor 9 on immune cells, trigger an immunomodulatory cascade, induce a Th1 -biased immune milieu, and have great potential as an adjuvant in cancer vaccines. In this study, a green one-step synthesis process was adopted to prepare an amino-rich metal-organic nanoplatform (FN). The synthesized FN nanoplatform can simultaneously and effectively load model tumor antigens (OVA)/autologous tumor antigens (dLLC) and immunostimulatory CpG ODNs with an unmodified PD backbone and a guanine quadruplex structure to obtain various cancer vaccines. The FN nanoplatform and immunostimulatory CpG ODNs generate synergistic effects to enhance the immunogenicity of different antigens and inhibit the growth of established and distant tumors in both the murine E.G7-OVA lymphoma model and the murine Lewis lung carcinoma model. In the E.G7-OVA lymphoma model, vaccination efficiently increases the CD4(+), CD8(+), and tetramer(+)CD8(+) T cell populations in the spleens. In the Lewis lung carcinoma model, vaccination efficiently increases the CD3(+)CD4(+) and CD3(+)CD8(+) T cell populations in the spleens and CD3(+)CD8(+), CD3(-)CD8(+), and CD11b(+)CD80(+) cell populations in the tumors, suggesting the alteration of tumor microenvironments from cold to hot tumors.