Abstract
Cellular behaviors are significantly affected by cellular microenvironment, including mechanical supports, electrical and chemical cues, etc. Three dimensional conductive nanofibers (3D-CNFs) provide the capability to regulate cellular behaviors using mechanical, geometrical and electrical cues together, which are especially important in neural tissue engineering. However, very few studies were conducted to address combined effects of 3D nanofibrous scaffolds and electrical stimulation (ES) on cortical cell cultures. In the present study, polypyrrole (PPy)-coated electrospun polyacrylonitrile (PAN) nanofibers with a 3D structure were successfully prepared for the cortical cell culture, which was compared to cells cultured in the 2D-CNFs meshes, as well as that in the bare PAN nanofibers, both in 2D and 3D. While smooth PAN 3D nanofibers showed dispersive cell distribution, PPy coated 3D-CNFs showed clusters of cortical cells. The combined effects of 3D conductive nanofibers and ES on neurons and glial cells were studied. Different from previous observations on 2D substrates, pulsed electrical stimulations could prevent formation of cell clusters if applied at the beginning of culture, but could not disperse the clusters of cortical cells already formed. Furthermore, the electrical stimulations improved the proliferation of glial cells and accelerate neuron maturation. This study enriched the growing body of evidence for using electrical stimulation and 3D conductive nanofibers to control the culture of cortical cells, which have broad applications in neural engineering, such as implantation, biofunctional in vitro model, etc.