Abstract
The ultrasound-assisted extraction conditions of Thesium chinense Turcz. crude polysaccharide (TTP) were optimized, and a TTP sample with a yield of 11.9% was obtained. TTP demonstrated the ability to stabilize high-internal-phase oil-in-water emulsions with an oil phase volume reaching up to 80%. Additionally, the emulsions stabilized by TTP were examined across different pH levels, ionic strengths, and temperatures. The results indicated that the emulsions stabilized by TTP exhibited stability over a wide pH range of 1-11. The emulsion remained stable under ionic strengths of 0-500 mM and temperatures of 4-55 °C. The microstructure of the emulsions was observed using confocal laser scanning microscopy, and the stabilization mechanism of the emulsion was hypothesized. Soluble polysaccharides formed a network structure in the continuous phase, and the insoluble polysaccharides dispersed in the continuous phase, acting as a bridge structure, which worked together to prevent oil droplet aggregation. This research was significant for developing a new food-grade emulsifier with a wide pH range of applicability.