Abstract
The restoration of healthy human skin function in the early stages is contingent upon the effective healing of wounds. This process requires the highest quality of care. While previous research has examined the individual impacts of Mentha Piperita and clinoptilolite, this experiment demonstrates their synergistic effects, presenting a promising new approach to expedite wound recovery. The study employed a total of 60 male Wistar rats, which were randomly assigned to one of five experimental groups: a negative control group, a positive control group (phenytoin ointment), a clinoptilolite group, an M. Piperita group, and an M. Piperita + clinoptilolite group. All ethical considerations were upheld, and neck wounds were created. Histological examinations were conducted on days 4, 7, and 14. Additionally, collagen deposition, inflammation, and re-epithelialization scores were evaluated on day 14 using the scoring system developed by Abramov. Wound healing assays were conducted on days 4, 7, 10, and 14. A p-value of less than 0.05 was considered statistically significant. The M. Piperita + clinoptilolite group demonstrated a statistically significant increase in the number of fibroblasts and vessels, accompanied by a notable reduction in the inflammatory cell count, when compared to all other experimental groups within the wound site. Furthermore, all groups exhibited significantly elevated collagen deposition and re-epithelialization scores (P-value <0.05) and diminished inflammatory cell infiltration (though not reaching statistical significance) compared to the control group. Furthermore, the M. Piperita + clinoptilolite group exhibited a markedly reduced wound surface area and accelerated healing rate compared to all other groups on all experimental days. On day 14, the recovery percentage reached an impressive 99.75 ± 0.5. The combination of clinoptilolite and M. piperita exerts a significant influence on wound healing, as evidenced by the histopathologic parameters and clinical outcomes. This combination is more effective than the separate usage of either substance or phenytoin.