Abstract
In contrast to ensemble-averaged measurements, single-molecule experiments directly display the heterogeneity of molecular properties in space and time. In many complex systems, spatial heterogeneity is regularly accompanied by temporal or dynamic heterogeneity; if a property differs from molecule to molecule, it will often vary in time for one and the same molecule. In this short paper, we discuss a few examples of complex systems where dynamical heterogeneity was observed in single-molecule or single-particle optical signals. For single biomolecules, the first demonstration of dynamic heterogeneity in a single enzyme was provided by Xie and colleagues. Other examples are found in glassy systems, and very recently in the magnetic relaxation of single superparamagnetic nanoparticles. The ubiquity of this phenomenon suggests that, rather than an exception, dynamic heterogeneity is the rule in complex systems with multiple degrees of freedom.