Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids

动态全场光学相干断层扫描:视网膜类器官的 3D 实时成像

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作者:Jules Scholler #, Kassandra Groux #, Olivier Goureau, José-Alain Sahel, Mathias Fink, Sacha Reichman, Claude Boccara, Kate Grieve

Abstract

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function.

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