Conclusions
These findings indicate that labeling with Feridex does not affect the immunomodulatory, nor the neural transdifferentiation potential of MScs and therefore, Feridex may be used for the tracking of this type of stem cells in clinical applications, without compromising their major functional properties.
Results
In this study we investigated whether such labeling of MSCs with the commercially used para-magnetic material, Feridex, has any negative effect on the above mentioned functional properties of MSCs. We labeled human mesenchymal stem cells (hMSC) with poly-L-lysine coated Feridex(®) and evaluated their cellular differentiation and immunomodulatory properties, in vitro. In comparison with unlabeled cells, labeled hMSC exhibited normal adipogenic and osteogenic differentiation, but decreased chondrogenic differentiation. Regarding neural differentiation, labeled and unlabeled cells were similar in their ability to express neural-like and glial like surface proteins. Finally, both labeled and unlabeled MSCs exhibited a dose-dependent, significant blocking effect on the proliferation of healthy donors lymphocytes following mitogen stimulation. Conclusions: These findings indicate that labeling with Feridex does not affect the immunomodulatory, nor the neural transdifferentiation potential of MScs and therefore, Feridex may be used for the tracking of this type of stem cells in clinical applications, without compromising their major functional properties.