Abstract
Arabidopsis thaliana proteins NECROTIC SPOTTED LESIONS 1 ( NSL1 ) and CONSTITUTIVE ACTIVE DEFENSE 1 ( CAD1 ) have previously been linked to immunity against phytopathogens such as Pseudomonas syringae pv. tomato ( Pst ) DC3000 (Noutoshi et al. 2006; Tsutsui et al. 2008; Asada et al. 2011; Fukunaga et al. 2017; Holmes et al. 2021). Here, we used a yeast 2-hybrid (Y2H) approach to explore their potential to interact with Pst DC3000 effectors. We found that NSL1 , but not CAD1 , interacted with the Pst DC3000 effector HopM1. Although further experiments are needed to validate this interaction, our results suggest that NSL1 may be a host target of HopM1.