Abstract
INTRODUCTION: Aminopenicillins resistance among Campylobacter jejuni and Campylobacter coli strains is associated with a single mutation in the promoting region of a chromosomal beta-lactamase bla(OXA61), allowing its expression. Clavulanic acid is used to restore aminopenicillins activity in case of bla(OXA61) expression and has also an inherent antimicrobial activity over Campylobacter spp. Resistance to amoxicillin-clavulanic acid is therefore extremely rare among these species: only 0.1% of all Campylobacter spp. analyzed in the French National Reference Center these last years (2017-2022). MATERIAL AND METHODS: Whole genome sequencing with bioinformatic resistance identification combined with mass spectrometry (MS) was used to identify amoxicillin-acid clavulanic resistance mechanism in Campylobacters. RESULTS: A G57T mutation in bla(OXA61) promoting region was identified in all C. jejuni and C. coli ampicillin resistant isolates and no mutation in ampicillin susceptible isolates. Interestingly, three C. coli resistant to both ampicillin and amoxicillin-clavulanic acid displayed a supplemental deletion in the promoting region of bla(OXA61) beta-lactamase, at position A69. Using MS, a significant difference in the expression of Bla(OXA61) was observed between these three isolates and amoxicillin-clavulanic acid susceptible C. coli. CONCLUSION: A combined genomics/proteomics approach allowed here to identify a rare putative resistance mechanism associated with amoxicillin-clavulanic acid resistance for C. coli.