Background
The global market for lactic acid is witnessing growth on the back of increasing applications of lactic acid for manufacturing polylactic acid. Indeed, the lactic acid market is expected to reach 9.8 billion US dollars by 2025. The new concept of meta-fermentation has been proposed in recent years as an alternative to fermentation with pure cultures, due to multiple advantages such as lower susceptibility to contamination, no need for sterilization of culture media or lower raw material costs. However, there are still challenges to overcome to increase the conversion efficiency, decrease formation of by-products and facilitate fermentation control. In this context, the
Conclusions
The operating conditions that enhanced the production of lactic acid from mixed cultures were 55ºC, 2 days HRT and pH 4.8-5.7, with pH-control once a day. The bioaugmentation strategy improved the results obtained in the prototype without applying reinoculation. Lactic acid was the main product along with other carboxylic acids. Further improvements are needed to increase purity as well as lactic acid concentration to reach economic feasibility of the whole process (digestion of OFMSW and downstream).
Results
A LAB-rich inoculum was generated with LAB isolated from the digestate and grown in the laboratory with MRS medium. After feeding this inoculum to the digester (bioaugmentation), lactic acid accumulation up to 41.5 gO2/L was achieved under optimal operating conditions. This corresponds to more than 70% of the filtered COD measured in the digestate. The amount of lactic acid produced was higher than the volatile fatty acids under all feeding strategies applied. Conclusions: The operating conditions that enhanced the production of lactic acid from mixed cultures were 55ºC, 2 days HRT and pH 4.8-5.7, with pH-control once a day. The bioaugmentation strategy improved the results obtained in the prototype without applying reinoculation. Lactic acid was the main product along with other carboxylic acids. Further improvements are needed to increase purity as well as lactic acid concentration to reach economic feasibility of the whole process (digestion of OFMSW and downstream).