Factors influencing the assessment of lung function in mice with influenza-induced lung disease

The constant-phase model (CPM) is commonly fit to respiratory system input impedance (Zrs) to estimate lung mechanics. Driving signal frequencies and the method of model fitting may influence the

For influenza-infected mice, the unweighted fit was poor, and airway resistance (Raw) was often biologically impossible. The weighted fit provided more realistic estimates of Raw. Different model fits and minimal frequencies had little effect on tissue mechanics.

BALB/c mice infected with influenza (or control) were challenged with methacholine. The CPM was fitted to Zrs, measured between 0·25 and 19·625 Hz, using both unweighted and weighted fits. The effect of different lowest frequencies was assessed.

To illustrate the effects of different CPM fits to Zrs data using a mouse model of influenza-induced lung disease.