Abstract
In this study, water-soluble and alkali-soluble cell wall polysaccharides were obtained from fruiting body extracted residual micropowders of Hericium erinaceus, harvested at seven different growing stages. The structural properties and in vitro immunity activities of cell wall polysaccharides extracted successively by hot water and sodium hydroxide solution were studied, and the results indicated that the yield and content of polysaccharides increased during the reproductive growth stage and decreased with the maturity of the fruiting body. Water-soluble cell wall polysaccharides mainly composed of glucose and galactose at a molar ratio of 3.4⁻14:1.0, and also contained a small ratio of glucuronic acid. The alkali-soluble cell wall polysaccharides were glucans with lower molecular weight and higher macrophage activation activity in vitro than water-soluble ones. Our findings suggest that the growth stages (H4 and H5) are suitable for harvesting H. erinaceus fruiting bodies with higher cell wall polysaccharide yield and functional benefits.