Aim
Despite intensive research during the last decade, it remains challenging to prepare extracellular vesicles (EVs) of high purity, especially from primary body liquids or protein-rich conditioned media. For now, time-consuming combinations of at least two orthogonal
Conclusion
Our results qualify FFE as a feasible, quick and reproducible technology for the preparation of bona fide EVs.
Methods
Exemplarily, conditioned media of mesenchymal stem/stromal cells raised in the presence of EV-containing 10% human platelet lysate were processed. We analyzed the obtained fractions by different technologies, including imaging flow cytometry, western blot and nanoparticle tracking analysis.
Results
We demonstrate that FFE quickly and reproducibly separates EVs from a huge proportion of molecules included in the original sample.