Abstract
OBJECTIVE: To evaluate the antibacterial efficacy of neem and cinnamon extract-mediated AgNPs against Enterococcus faecalis and assess their cytotoxicity. MATERIALS AND METHODS: Neem (Azadirachta indica) and cinnamon (Cinnamomum verum) extracts were employed to reduce silver nitrate, synthesizing AgNPs, with visual changes confirming the reaction. Antibacterial properties were assessed using Mueller-Hinton agar for zone of inhibition (ZOI) and minimal inhibitory concentration (MIC) assays. Cytotoxicity was evaluated on nauplii models. RESULTS: Neem-based AgNPs exhibited significantly larger ZOIs compared to cinnamon-based AgNPs against E. faecalis (P < 0.05). MIC assays demonstrated both bacteriostatic and bactericidal effects. Cytotoxicity results showed acceptable safety profiles for both AgNP types. CONCLUSION: Neem and cinnamon extracts are viable agents for green AgNP synthesis, with neem-based AgNPs showing promising antibacterial efficacy against E. faecalis.