Conclusions
In conclusion, we provide evidence that CK10, CK13, and CK14 are expressed on the protein level in different urinary bladder transitional cell carcinoma cell lines, but that their targeting does not affect the viability of the carcinoma cells.
Material and methods
We analyse the expression of CK10, CK13, and CK14 in 4 different urinary bladder transitional cell carcinoma cell lines via western blot. Furthermore, we downregulate the expression of CK13 and CK14 using siRNAs and evaluate the cell viability of the carcinoma cells.
Methods
We analyse the expression of CK10, CK13, and CK14 in 4 different urinary bladder transitional cell carcinoma cell lines via western blot. Furthermore, we downregulate the expression of CK13 and CK14 using siRNAs and evaluate the cell viability of the carcinoma cells.
Results
In this study, we show that different urinary bladder transitional cell carcinoma cell lines have distinct expression pattern of the keratins CK10, CK13, and CK14. Using several siRNAs targeting either CK13 or CK14, we show that both keratins have long protein half-lives. Although we achieve a reduction in CK13 and CK14 protein levels, these reductions do not influence the cell viability of the cell lines. Conclusions: In