Human ex vivo dentin-pulp complex preservation in a full crown model

全冠模型中人类离体牙本质牙髓复合体的保存

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作者:João Botelho, Maria Alzira Cavacas, Gonçalo Borrecho, Mário Polido, Pedro Oliveira, José Martins Dos Santos

Conclusions

This study showed that it is possible to preserve a living dentin-pulp complex after tooth extraction during 7 days.

Methods

Twenty intact third molars, after preparation, were divided into four groups, with five samples each. We placed the negative control samples (C) in saline, and the tested groups were placed (T) in supplemented DMEM, at two different times: 1 and 7 days. The specimens were processed for light microscopy observation.

Results

Contrary to C-groups, T-groups showed a functional dentin-pulp complex. The treated dentin-pulp complex presents normal histological appearance. Conclusions: This study showed that it is possible to preserve a living dentin-pulp complex after tooth extraction during 7 days.

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