kin-3 genetically suppresses sur-6 in centrosome assembly during Caenorhabditis elegans embryogenesis

在秀丽隐杆线虫胚胎发生过程中,kin-3基因通过抑制sur-6参与中心体组装。

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Abstract

Protein phosphorylation plays a critical role in cell cycle progression. In Caenorhabditis elegans , Casein Kinase II (CK2) negatively regulates centrosome assembly, and Protein Phosphatase 2A (PP2A) (SUR-6 /B55) acts as a positive regulator of centrosome duplication, suggesting CK2 and PP2A (SUR-6 /B55) play opposing roles in centrosome assembly. Here, we examined the genetic interaction between kin-3 , encoding the catalytic subunit of CK2, and sur-6 , encoding the PP2Aregulatory subunit SUR-6 /B55, in Caenorhabditis elegans embryos. Our results show that kin-3 (RNAi) partially restores normal centrosome duplication and embryonic viability to hypomorphic sur-6 mutants, suggesting that kin-3 genetically suppresses sur-6 in centrosome assembly during Caenorhabditis elegans embryogenesis.

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