Abstract
Bisphenol A (BPA) is a synthetic estrogen widely used in the manufacture of food packaging materials, raising concerns due to its potential migration into food products. This study aims to determine BPA levels in pasteurized milk marketed in Algeria, using an easy-to-handle and efficient liquid-liquid extraction method coupled with liquid chromatography and fluorescence detection. A total of 30 pasteurized milk samples packaged in plastic were analyzed. The method validation demonstrated excellent linearity, with a limit of detection of 3.76 µg/L and a limit of quantification of 11.40 µg/L. Among the analyzed samples, 17 contained detectable BPA levels, ranging from not detectable to 24.07 µg/L, with an average concentration of 3.77 ± 5.77 µg/L, compliant with European regulation. The health risk assessment, based on estimated chronic daily intake and hazard index, indicated no significant risk associated with BPA exposure through milk consumption in the studied population. Additionally, the estrogenic equivalence of BPA in milk was 6.032 × 10(-5) µgE(2)/L, confirming a low estrogenic activity.