Abstract
Molecular-based detection of pathogens from potato tubers hold promise, but the initial sample extraction process is labor-intensive. Developing a robotic tuber sampling system, equipped with a fast and precise machine vision technique to identify optimal sampling locations on a potato tuber, offers a viable solution. However, detecting sampling locations such as eyes and stolon scar is challenging due to variability in their appearance, size, and shape, along with soil adhering to the tubers. In this study, we addressed these challenges by evaluating various deep-learning-based object detectors, encompassing You Look Only Once (YOLO) variants of YOLOv5, YOLOv6, YOLOv7, YOLOv8, YOLOv9, YOLOv10, and YOLO11, for detecting eyes and stolon scars across a range of diverse potato cultivars. A robust image dataset obtained from tubers of five potato cultivars (three russet skinned, a red skinned, and a purple skinned) was developed as a benchmark for detection of these sampling locations. The mean average precision at an intersection over union threshold of 0.5 (mAP@0.5) ranged from 0.832 and 0.854 with YOLOv5n to 0.903 and 0.914 with YOLOv10l. Among all the tested models, YOLOv10m showed the optimal trade-off between detection accuracy (mAP@0.5 of 0.911) and inference time (92 ms), along with satisfactory generalization performance when cross-validated among the cultivars used in this study. The model benchmarking and inferences of this study provide insights for advancing the development of a robotic potato tuber sampling device.