Evaluation of immunomagnetic separation for recovery of Cryptosporidium parvum and Giardia duodenalis from high-iron matrices

免疫磁分离从高铁基质中回收小隐孢子虫和十二指肠贾第鞭毛虫的评估

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作者:G P Yakub, K L Stadterman-Knauer

Abstract

In this study we examined the recovery of Cryptosporidium parvum and Giardia duodenalis from matrices containing various concentrations of dissolved iron. The organisms were recovered by using the immunomagnetic separation-immunofluorescent assay method, and the levels of recovery were compared to the dissolved iron concentrations. The levels of recovery of C. parvum decreased sharply at dissolved iron concentrations greater than 4 mg/liter, while the levels of recovery of G. duodenalis decreased sharply at concentrations greater than 40 mg/liter.

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