Abstract
In this study, we aimed to clarify the taxonomic positions of Micromonospora veneta DSM 109713(T) and Micromonospora coerulea JCM 3175(T) using whole-genome phylogenetic analysis, pangenomic analysis and chemotaxonomic characteristics. The 16S rRNA gene sequences of veneta DSM 109713(T) and M. coerulea JCM 3175(T) were highly similar (99.2%), and in the phylogenetic trees constructed using whole genome sequences and 16S rRNA gene sequences, M. veneta DSM 109713(T) and M. coerulea JCM 3175(T) clustered together and formed a clade. The values of average amino acid (AAI), ANI-BLAST (ANIb) and ANI-MUMmer (ANIm), and digital DNA-DNA hybridization (dDDH) between M. veneta DSM 109713(T) and M. coerulea JCM 3175(T) were 97.57%, 97.81%, 98.40%, 85.0%, respectively, which are all greater than the thresholds used for species delimitation (AAI: 95.5%, ANI: 95-96%, and dDDH: 70%). Most phenotypic and chemotaxonomic features between the two species were similar, with only a few differences. Based on the combined evidence from genomic and phenotypic comparison, we propose Micromonospora veneta as a later heterotypic synonym of Micromonospora coerulea.