Comparison of cultures from rectoanal-junction mucosal swabs and feces for detection of Escherichia coli O157 in dairy heifers

直肠肛门连接处粘膜拭子和粪便培养物对奶牛大肠杆菌 O157 的检测比较

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作者:Margaret A Davis, Daniel H Rice, Haiqing Sheng, Dale D Hancock, Thomas E Besser, Rowland Cobbold, Carolyn J Hovde

Abstract

Fecal culture for Escherichia coli O157:H7 was compared to rectoanal mucosal swab (RAMS) culture in dairy heifers over a 1-year period. RAMS enrichment culture was as sensitive as fecal culture using immunomagnetic separation (IMS) (P = 0.98, as determined by a chi-square test). RAMS culture is less costly than fecal IMS culture and can yield quantitative data.

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