Abstract
Alcohol use disorder is characterized by persistent drinking in the face of negative consequences. Such inflexible drinking requires dorsolateral striatum fast-spiking interneurons, which comprise roughly 1% of all striatal neurons. How chronic ethanol exposure affects fast-spiking interneuron physiology is poorly understood. We discover in mice that chronic ethanol exposure induced a dramatic loss of GABAergic, but not glutamatergic, synapses onto dorsolateral striatum fast-spiking interneuron somata and proximal dendrites where perineuronal nets, a subdivision of the extracellular matrix, are enriched. We found that chronic ethanol exposure degraded these perineuronal nets and that enzymatically degrading perineuronal nets similarly reduced GABAergic transmission onto dorsolateral striatum fast-spiking interneurons. Modeling the effect of alcohol, we find that silencing extrinsic GABAergic projections to the dorsolateral striatum increased voluntary ethanol consumption. Taken together, these data suggest chronic alcohol exposure remodels perineuronal nets and inhibitory synapses on fast-spiking interneurons to facilitate alcohol drinking.