Abstract
BACKGROUND: This study aims to formulate and evaluate the staining effect of three different types of mouthwash containing nanoparticles (NPs) and compare them with chlorhexidine (CHX) mouthwash on dental enamel. MATERIALS AND METHODS: Twenty intact premolars were randomly assigned to four groups. The specimens were then immersed in CHX mouthwash (Group I), colloidal solutions containing cerium oxide NPs (Group II), Glycyrrhiza glabra NPs (Group III), and a cerium oxide NP and G. glabra NP composite (Group IV). The baseline color determination was made (T1) using a digital spectrophotometer. After 24 h, the shade of the samples was recorded (T2). The third color assessment was accomplished on the 15(th) day (T3) and 30(th) day (T4). RESULTS: The Kolmogorov-Smirnov test for normality was performed on four groups. One-way ANOVA revealed no significant differences in the ∆ E between groups at various time intervals. On the 1(st) day, the highest staining was found in Group IV, and the least staining was found in Group I. On the 15(th) day, the highest staining was found in Group III, and the least staining was found in Group II. On the 30(th) day, the highest staining was found in Group III, and the least staining was found in Group II. P > 0.05, indicating that the results show no significant difference. CONCLUSION: Cerium oxide NPs containing mouthwash produced equal enamel discoloration as compared with CHX, which can be used as an alternative to CHX.