Abstract
Malignant edema is a severe and rapidly fatal disease that affects both domestic and wild livestock. The disease manifests following the introduction of Clostridium spp. into wounds or skin damage, with Clostridium septicum being commonly linked with malignant edema. This disease, characterised by oedema, doughy swelling and skin necrosis, is underreported in Iran, leading to a lack of awareness among clinicians. Addressing this issue is imperative, as evidenced by current research efforts aimed at enhancing our understanding of the disease's prognosis, bacteriological and molecular diagnosis, clinical signs, and treatment.The present study was initiated after the detection of suspicious signs of malignant edema in three separate flocks with imported breeds. Investigations included regular clinical exams and sample collection from subcutaneous tissue. The affected livestock consists of five Île-de-France sheep and two Romane rams, with one Île-de-France ram succumbing to the disease.The bacteriological procedure, including Gram staining and isolation of the causative agent, was meticulously carried out using the standard method.The PCR assay was conducted to validate the existence of C. septicum and reject the presence of Clostridium chauvoei by employing specific primers. The diagnosis of malignant edema in the affected sheep was confirmed through clinical, macroscopic, and bacteriological examinations, all of which corroborated the presence of C. septicum.The PCR assay demonstrated the presence of the C. septicum, thereby verifying the bacteriological procedure.Initial signs of the infection included depression, weakness, high fever, and colic, followed by regional pain, crepitation, swelling characterised by a doughy consistency, edema, pain, and necrosis. The study emphasises the significance of early diagnosis and antibiotic intervention (Penicillin and Streptomycin) in preventing fatalities due to malignant edema. Nevertheless, it is important to note the persistent challenge of the inability to repair necrotic tissue at the lesion site. Malignant edema, not being a prominently warned disease and with vaccinations available against its causative agent, has received comparatively less focus from clinicians and researchers in Iran.