Detection of Salmonella strains and Escherichia coli O157:H7 in feces of small ruminants and their isolation with various media

检测小反刍动物粪便中的沙门氏菌和大肠杆菌 O157:H7 并用不同培养基进行分离

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作者:Steven Pao, Dhartika Patel, Aref Kalantari, Joseph P Tritschler, Stephan Wildeus, Brian L Sayre

Abstract

Salmonella strains and Escherichia coli O157:H7 were detected in 17 and 5 small ruminants in Virginia, respectively, of 287 tested. Background microflora interfered with the fecal analysis. The combination of Salmonella enzyme immunoassay (EIA) detection and xylose-lysine-deoxycholate agar isolation was satisfactory. Modifying enrichment to a 1:100 dilution enabled effective E. coli O157:H7 detection by EIA and isolation by sorbitol-MacConkey agar with cefixime-tellurite.

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