Abstract
A sustainable hybrid material based on carbonized cotton cloth/zeolite imidazolate framework-71/Fe(3)O(4)/polythionine (CC/ZIF-71/Fe(3)O(4)/PTh) was synthesized and applied in ultrasound-assisted dispersive magnetic solid-phase extraction (USA-DMSPE) followed with high performance liquid chromatography-ultraviolet detection (HPLC-UV) for simultaneous quantitation of diclofenac (DIC) and febuxostat (FEB) in human plasma. The surface of CC was modified with nanoarrays of zeolite imidazolate framework-71/Fe(3)O(4)/Polythionine. At first, an in situ synthesis of ZIF-71 in the presence of CC was carried out, and followed with magnetization process and oxidative polymerization of thionine. The nano-modifier agents improved the merits of the sorbent involving stability, porosity, mast transfer, π-π interactions and selectivity of CC. Characterizations of the hybrid sorbent were examined with different instrumental techniques. The limits of detection (LODs, S/N = 3) were recognized 2.1 ng mL(-1) for DIC and 3.7 ng mL(-1) for FEB. Acceptable linearity (0.992 ≤ r (2) ≤0.996) and relatively broad dynamic ranges of 10.0-1800.0 ng mL(-1) and 15.0-2500.0 ng mL(-1) were achieved for DIC and FEB, respectively. Reasonable intra-assay (≤7.2%, n = 9) and inter-assay (≤7.5%, n = 9) precisions as well as appropriate accuracies (≤8.0%) were provided illustrating applicability of the current approach for analytical purposes. Eventually, CC/ZIF-71/Fe(3)O(4)/PTh was employed as four-part sorbent for the assessment of DIC and FEB in human plasma at trace levels and subsequently main pharmacokinetic data such as T (1/2), T (max,) C (max), and AUC(0-24) of these drugs were comprehensively investigated.