Computing Arithmetic Functions Using Immobilised Enzymatic Reaction Networks

利用固定化酶反应网络计算算术函数

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作者:Nikita M Ivanov, Mathieu G Baltussen, Cristina Lía Fernández Regueiro, Max T G M Derks, Wilhelm T S Huck

Abstract

Living systems use enzymatic reaction networks to process biochemical information and make decisions in response to external or internal stimuli. Herein, we present a modular and reusable platform for molecular information processing using enzymes immobilised in hydrogel beads and compartmentalised in a continuous stirred tank reactor. We demonstrate how this setup allows us to perform simple arithmetic operations, such as addition, subtraction and multiplication, using various concentrations of substrates or inhibitors as inputs and the production of a fluorescent molecule as the readout.

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