Abstract
This study presents the development of a miniaturized device for supercritical fluid chromatography coupled with mass spectrometry. The chip-based, modular nanoSFC approach utilizes a particle-packed nanobore column embedded between two monolithically structured glass chips. A microtee in the pre-column section ensures picoliter sample loads onto the column, while a microcross chip structure fluidically controls the column backpressure. The restrictive emitter and the minimal post-column volume of 16 nL prevent mobile phase decompression and analyte dilution, maintaining chromatographic integrity during transfer to the atmospheric pressure MS interface. This facilitates high-speed chiral separations in less than 80 s with high reproducibility.